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1. The Diversity, Distribution And Phenetic Relationships Of Herpetiles Of District Kasur

by Waqas Ali (2008-VA-609) | Dr. Arshad Javid | Ms. Sana Ashraf | Prof. Dr. Muhammad Ashraf.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Amphibians and reptiles (herpetiles) are cold-blooded animals. Mostly lay eggs, fertilization is external in amphibians and internal in reptiles. Amphibians undergo metamorphosis to reduce competition between larval (usually aquatic) and adult (terrestrial) stage. The reptiles are found in a variety of habitats throughout the world except some isolated islands. Diversity and abundance of herpetiles is dependent on climate and geographic position of any region and is also directly linked with some avian and mammalian species. Amphibians and reptiles are important bio-indicators of climate change and the survival of both taxa is under continuous threat due to deforestation, habitat loss, fragmentation, urbanization and pollution. Data on local distribution patterns is helpful in regional conservation planning (Petrov 2004). Herpetiles are the important members of environment and have important position in pyramids. They control the population of many insects and pests, and themselves are source of food for many predators. Amphibians and many reptiles transfer nutrients from aquatic to terrestrial ecosystems and if they are removed from any ecosystem, the algae communities, invertebrate populations, predator dynamics, leaf litter decompositions and nutrient cycling can be affected (Baig 2006 ). Pakistan has the world’s rarest animals and plants but these are now in danger due to habitat loss, overuse and anthropogenic activities. The herpetofauna of Pakistan is represented by 219 species; 24 species of amphibians and 195 species of reptiles out of these 9 species of amphibians and 13 species of reptiles are endemic to Pakistan (Khan 2004). The herpetiles received less attention of scientific community hence these taxa remained unexplored in Pakistan. There is extreme scarcity of data on the distribution of various amphibian and reptilian species in the country (Khan 2006). The only authentic source of information till so far on herptiles in this region is the data collected and compiled by Daniel and Khan (2002, 2006). In Pakistan arid to semi-arid climatic conditions prevail that make it, an amphibian poor country however, humid riparian conditions in the Indus Valley, streams in the northern Himalayan sub-mountainous region and water channels in the western Baluchistan highland are home to some 24 amphibians, that belong to four families viz. Bufonidae, Megophryidae, Microhylidae and Ranidae (Khan 2011). The reptilian fauna of Pakistan is represented by 195 species belonging to 23 families viz. Cheloniidae, Dermochelyidae, Emydidae, Testudinidae, Trionychidae, Crocodylidae, Gavialidae, Agamidae, Chameleonidae, Eublepharidae, Gekkonidae, Lacertidae, Scincidae, Uromastycidae, Varanidae, Leptotyphlopidae, Typhlopidae, Boidae, Colubridae, Elapidae, Hydrophiidae, Viperidae and Crotalidae (Khan 2004). In Pakistan, due to over hunting the marsh crocodile is in danger and nearly extinct. Now this species only occur in small numbers in Sindh and a few areas in Balochistan. The gharial is now few in numbers and found only between the Sukkur and Guddu barrages. In addition monitor species are heavily hunted for their skins. 72 snake species found in Pakistan and out of these, 14 marine and 12 terrestrial snake species are poisonous; most well-known are the Indian cobra, common krait, saw-scaled viper and Russel's viper. The snake venom is a complex mixture of enzymes including the pro-coagulant, non-enzyme proteins, peptides, carbohydrates, amines, lipids and metal ions. The venom exertsneuro-toxic, cytotoxic and hemotoxic effects. Genus monospecific Teratolepsis and Eristicophis is endemicto Pakistan. The Chagai Desert is of particular interest for reptiles, with six species of reptiles including five lizards and one snake are endemic to Pakistan and a further six species found only here and along the border of Iran. Important populations of marine turtles nest on Pakistan's southern beaches. The internationally threatened species of reptiles in Pakistan are Green and olive turtle, mugger, gharial, central Asian monitor, Indian python, central Asian cobra (Groombridge 1988). District Kasur is located between two rivers of the Punjab province, namely river Ravi and Sutlej. This district is bounded by India from east, Okara from south, Sheikhupura from north-west and Lahore covers its north side. District Kasur is administratively divided in to four Tehsils i.e. Kasur, Chunian, Pattoki and KotRadhaKishan. The district lies 150 to 200 m above sea level and experiences extreme hot weather during summer (April -September) to severe cold in winter (November to February). The difference between day and night temperatures is considerable. Average annual rainfall is 500 mm. Water logging and salinity has affected large area of the district making underground water brackish (Anwar 2012). Reptiles and amphibians have historically taken less concern as components of many ecosystems. Many ecosystems support high densities of herpetofaunaas compared to endothermic vertebrates because they make efficient use of energy (Pough 1980).Moreover, high densities of reptiles and amphibians presentin many ecosystems provide major role for the trophic transfer of energy and matter except one study that shows high amphibian densities affect ecosystem processes (Wyman 1998). Moreover, the high rate of herpetofauna decline increase the interest in scientific communities for documenting the abundance and status of amphibians on a global scale (Collins and Storfer 2003). Despite the high densities of herpetiles present in many ecosystems, it is difficult to sample them quantitatively because many snakes and several species of amphibians and lizards live in burrows making their capture difficult (Conant and Collins 1998). Furthermore, the cryptic nature of herpetiles, hibernation in winter, climatic factors affects the activity and the presence or abundance of amphibians and reptiles species (Zug et al 2001).As a result, many methods have been developed to sample herpetofauna such as drift fences with pitfall and funnel traps are a good way to maximize the number of individuals and number of herpetofauna species captured (Gibbons and Semlitsch, 1982; Enge 2001). On the other hand, drift fence surveys can be time-intensive and inappropriate applications can result in low capture rates of some species or high mortality of captured animals. Moreover, documenting the presence of all species occurring in a given area is difficult, if not impossible, and can be particularly time-intensive in systems with many rare species (Dodd 1991). Herpetological studies carried out in Pakistan are outdated and are mainly limited to Sind and Baluchistan provinces (Minton 1966; Mertens 1969). There is paucity of data on herpetiles of Punjab, densely populated province of the country. There is dire need to explore herpetofauna of the province in general and the district kasur is specific due to geographic location and climatic condition. Present study was therefore planned to explore herpetofauna diversity and distribution patterns of various amphibians and reptiles inhabiting district Kasur. Availability: Items available for loan: UVAS Library [Call number: 2215,T] (1).

2. Studies On Growth Performance, Morphology, Reproductive Traits And Behavioral Aspects Of Ring Necked Pheasants In Captivity

by Sana Ashraf | Dr. Arshad Javid | Dr. Khalid Mehmood Anjum | Prof. Dr. Muhammad Ashraf | Prof. Dr. Muhammad Akram.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Besides ecological importance, pheasants also have aesthetic values which mainly contribute to their decline in population due to poaching in their native habitat. Among 49 species of pheasants in the world, 5 are endemic to Pakistan with distribution in the Himalaya and remote northern parts of the country. Due to increase in human population, intrusion, poaching, and habitat disturbance pheasants are threatened. However, much of the work on captivity is based on production rather morphological, ornamentation or reproductive traits which could have important implications for the management of wild and captive populations as a whole. Some studies still remain to be explored. Present study was conducted to find out the relationship of egg weight with egg quality parameters and growth traits of ring-necked pheasant Phasianuscolchicus. Total of 450 eggs were collected and were divided into three egg weight categories viz. light (20.0-26.0g), medium (27.0-32.0g) and heavy (33.0-40.0g) egg weight eggs. Fifty eggs for each of the egg category were reserved for the evaluation of internal egg quality parameters. External egg quality parameters i.e. egg length, breadth, egg volume and surface area varied significantly (P<0.05) between all the three egg weight categories. Similarly, significantly higher albumen and yolk weight were recorded in heavy weight egg category while non-significant relationship of egg weight was observed for shell and membrane thickness, yolk percentage, yolk index, yolk pH and albumen pH of the egg. The hatching percentage for the remaining 300 egg kept in incubator was 47.33%. Forty chicks from each of the egg weight category were selected and chick weight, wing length and wingspan were taken at the time of hatching and thereafter increase in these parameters were noted on weekly basis. The effect of egg weight on chick weight, live weight gain, wing length and wingspan was significant (P<0.05) from 1st to 12th month of age. Our studies revealed that egg weight has strong influence on external and internal characteristics of the eggs and the growth parameters in P. colchicus chicks. The present study was planned to evaluate the time budgets of ring necked pheasants Phasianuscolchicus in captivity. The birds were kept in cages of 5 ft × 5 ft × 3 ft (length × width × height) and were housed in a 20 ft × 20 ft (length × width) well ventilated room at Department of Wildlife and Ecology, Ravi Campus, University of Veterinary and Animal Sciences, Lahore. Captive birds were divided into three categories viz. adult male, adult female and chicks and were placed into separate cages. Behavioral parameters viz. jumping, aggression, preening, feather pecking, walking, standing, sitting, litter pecking, drinking, feeding, body shaking, voice call and feather flapping were assessed for 30 birds from each of the three categories through scan sampling. Statistically significant variations were recorded in behavioral aspects among all the three categories. Male birds spent significantly higher times in aggression (155.26±3.10 sec), preening (74.04±3.05 sec), walking (1370.93±54.45 sec), drinking (74.00±3.18 sec), body shaking (24.92 ±3.11 sec), voice call (20.08±3.17 sec) and feather flapping (15.42±2.73 sec) while female P. colchicus spent significantly higher times in sitting (364.57±3.74 sec). Similarly, the chicks spent significantly higher times in jumping (36.17±2.75 sec), feather pecking (265.19±3.17 sec), standing (1230.13±23.86 sec), litter pecking (234.89±2.97 sec) and feeding (115.44±3.11 sec) as compared to the adult female and male birds. Fecal and blood samples of ring necked pheasants, Phasianuscolchicus were analyzed to record the parasitic prevalence in these pheasants. A total of 1000 samples, 500 blood and 500 fecal samples were collected from Captive Breeding Facilities for Birds, Department of Wildlife and Ecology, Ravi Campus, University of Veterinary and Animal Sciences, Lahore. Parasitic genera identified from blood samples of P. colchicus include Leukocytozoon, Plasmodium and Haemoproteus. Prevalence of Leukocytozoon was 16 % while the prevalence of Haemoproteus was 14.3%. Parasitic genera identified from fecal samples of P. colchicus include Eimeria, Isospora, Trichomonas and Giardia. Eggs of five species of nematodes viz. Capillaria, Syngamus trachea andAscaridia,Heterakisisoloncheand Heterakisgallinarum were also identified from the fecal samples. The ectoparasites include one species of burrowing mite Knemidocoptesmutansand two species of chewing lice i.e. Amyrsideaperdicis and Lipeurusmaculosus. Variations in hematological parameters during different life history stages were recorded in ring-necked pheasants (Phasianuscolchicus) for a period of 1 year. Thirty birds were selected for analysis of selected hematological parameters viz. red blood cells count, white blood cells count, hemoglobin, mean corpuscular hemoglobin concentrations, total serum protein and leucocyte count. These birds were kept in cages, each cage having separate drinking and feeding facilities. Five birds per cage were confined and these cages were housed in a well-ventilated 20 × 20 feet (length × width) room. Blood samples were taken from ulnar vein and variations in blood parameters were recorded on monthly basis. Significantly, lower RBC’s count was observed during 2nd month of age while during same month significantly higher WBC count was noticed. Significantly lower values of hemoglobin were observed during 1st and 2nd month of age. Significantly higher mean corpuscular hemoglobin concentration percentage (MCHC%) was recorded during 6th and 7th month of age. Lower packed cell volume (PCV) values were observed during 2nd and 6th month of age while the total serum protein concentrations were recorded maximum during 10th month of age. Significantly, higher heterophils count was recorded during 2nd and 10th month of age while maximum lymphocyte count was observed during 2nd and 7th months of age. Significantly, higher concentrations of monocytes were recorded during 11th, 12thand 10th month of age. The eiosinophils count varied from minimum (110 ± 13.50) during 3rd month to maximum (902 ± 93.22) during 11th month of age. Similarly, significantly higher values of basophils were recorded during 1st month of age. It can be concluded from the present study that the blood profile of the pheasants changes with age. Variations in hematological parameters for adult male and female ring-necked pheasants (Phasianuscolchicus) were recorded. Forty adult ring-necked pheasants (20 ♂, 20 ♀) were kept in separate cages, each cage having separate drinking and feeding facilities. Five birds per cage were confined and these cages were housed in a well-ventilated 20 × 20 feet (length × width) room. Blood samples were taken from ulnar vein. Different blood and serum chemistry parameters such as red blood cells (RBCs), white blood cells (WBCs), hemoglobin (Hb ) concentrations, mean corpuscular hemoglobin concentration (MCHC), packed cell volume (PCV), heterophils, lymphocytes, monocytes, eosinophils, basophils, ALP, uric acid, cholesterol, total serum protein, albumin and creatinine were determined among adult male and female pheasants. Non-significant differences in RBCs, WBCs, heterophils, eosinophils, MCHC and Hb values were observed among male and female pheasants. Significantly, higher values of lymphocytes, monocytes and PCV were observed in males while higher basophil count was observed in female as compared to male birds. Significantly higher values for ALP, cholesterol, total serum protein and creatinine were observed in males while higher uric acid values were observed in females as compared to male P. colchicus. However, non-significant differences in albumin were recorded among male and female birds. Availability: Items available for loan: UVAS Library [Call number: 2350-T] (1).

3. Welfare Assessment Of Wild Turkeys (Meleagris Gallopavo) Subjected To Free Range, Semiintensive And Confinement Rearing Systems

by Irfan (2008-VA-724) | Dr. Arshad Javid | Prof. Dr. Muhammad Ashraf | Prof. Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: The volume of turkey production is as small compared to the broiler production, although, the turkey industry has attained a significant increase since 1980, rising from 122 million to 226 million turkeys by 2006 within the European Union, while values of turkeys produced in the US during 2010 was $4.37 billion. The s scientific literature about welfare of confinedly reared turkeys is scarce compared to other poultry birds. There is a need for more insight into the factors that enhance turkey welfare, not only to sustainable production of turkey meat where the public demands s management practices for the welfare of turkeys, but also because the information is needed to decrease losses due to poor performance of the birds. Besides ecological importance, Meleagris gallopavo also have aesthetic values which mainly contribute to decline in population of turkeys due to poching in their native habitat. Demand for poultry meat is increasing throughout the world, due to of its lower cost when compared to other meats such as pork and beef, and also lower fat content and excellent source of protein. The consumption of turkey meat is about 4.7 million metric tons annually throughout of the world and with the US accounting for 50% of all consumption. A total of 120, day-old turkey (Maleagris gallopavo) chicks were arranged into four groups. Four diets having varying levels of crude protein (CP) viz. 16% CP, 18% CP, 20% CP and 22% CP were prepared and were designated as control (T0), treatment 1 (T1), treatment 2 (T2) and treatment 3 (T3) diets, respectively. Group 1 birds were fed with T0 diets, group 2 with T1 diets, group 3 with T2 and group 4 birds were fed with T3 diets. Significantly higher weight gain 2950.86±1952.58g, body length 30.064±11cm, beak length 3.53cm, body girth 43.41±16.30cm, thigh length 18.18±6.47cm, sternum-length 11.98±4.70cm, wing-length 47.10±16.17cm, wingspan 101.36±34.32cm, shank-length and tarsus-length 13.78±4.47cm were Summary 90 recorded in M. gallopavo fed with T3 diets. Feed conversion ratios (FCR) for T0, T1, T2 and T3 diets were 2.296, 2.236, 2.099 and 1.934, respectively. Internal and external quality parameters of egg were also analyzed. The time budget of turkeys (Meleagris gallopavo) reared under free-range and confinement rearing systems was recorded and compared from day old chick to the sixth month of age. Throughout the study period, M. gallopavo reared under free-range rearing system spent relatively greater time litter pecking (23.51%) followed by walking (19.99%), feeding (16.33%), preening (13.72%), feather pecking (6.07%), aggression (5.94%), drinking (5.90%), immobility (2.36%), standing (2.29%) and jumping (1.96%). Similarly, the birds reared under confinement rearing system spent relatively greater time in lying (17.82%) followed by litter pecking (15.71), preening (12.93%), walking (11.47%), standing (8.35%), drinking (8.31%), aggression (6.85%), feeding (6.46%), feather pecking (6.04%), immobility (4.59%) and jumping (1.46%) behavior. It was observed during the present study that the birds reared under free-range rearing system spent significantly greater time in litter pecking, walking and feeding behaviors as compared to the birds reared under confinements. These behaviors are indicators of good health of the animals, therefore free-range system is recommended over confinement rearing system for farming of M. gallopavo. Growth performance, carcass yield and meat sensory quality attributes of turkeys Meleagris gallopavo reared under free-range and confinement rearing systems were compared. There were two treatments, i.e. indoor and free range, each containing 25 birds. In indoor treatment, the turkeys were raised in a 20 × 20 feet (length × width) well ventilated room while in free-range treatment, the birds were housed in an open shed having same dimensions i.e. 20 × 20 feet (length × width), however, an additional free-range grassy paddock was provided to Summary 91 them. To compare sensory attributes, 4 ready to-cook turkeys having equal size were selected from each the rearing systems, were skinned and boneless breast fillets and thighs were prepared, weighed and steam roasted. The roast breast meat of free-range turkeys was darker and yellower than thigh meat from indoor birds. Score for breast meat tenderness and appearance varied significantly (P<0.05) between free-range and confinement reared birds. However, nonsignificant differences were recorded for thigh meat tenderness between free-range and confined turkeys. Significantly higher (P<0.05) meat redness was recorded in thigh meat of turkeys reared in free-range system than thigh meat of confined M. gallopavo while non-significant differences in breast meat color were observed between free-range and confined birds. Significantly higher juiciness and oiliness was recorded for confined birds as compared to the free-range reared turkeys. Morphological characteristics of turkey Meleagris gallopavo semen were recorded and hatchability percentages of the artificially inseminated hens were assessed. To study the effect of natural mating on hatchability 15 (12♀, 3♂) M. gallopavo were randomly selected and both the genders were kept separately for two weeks, thereafter these birds were arranged in three groups, each containing one male and four female birds. For AI, male (n = 3) and female (n = 12) birds were kept separately through the experiment and female birds were inseminated on weekly basis. Male birds were conditioned and trained for semen collection through abdominal massage and morphological characteristics of the collected semen were recorded. Mean abnormal spermatozoa rate was 14.61±1.61% with most common acrosomal defects 36.67±3.80% followed by defects in mid-piece 8.59±0.24%, head defects 7.15±1.21% and tail defects 6.69±1.97%. Laid eggs were collected, stored and were incubated for hatching. Hatchability percentage in artificially inseminated hens was greater 88.85% than natural mating 82.35%. Summary 92 Variations in serum biochemical profile with increase in age were analyzed in turkeys (Meleagris gallopavo). Gender-wise variations and effect of rearing systems i.e. free range, semi-intensive and confinements were also assessed from the 1st to 6th month of age. Variations were recorded in serum biochemical profile with increase in age. Significantly (P<0.05) higher values of cholesterol 8.39±0.48 mmol/L, globulin 2.71±0.34 g/dL, albumin g/dL 3.82±0.58 g/dL, total protein 5.35±0.55 g/L, urea 26.95±0.65 mg/dL, creatinine 0.83±0.01 μmol/L, alanine aminotransferase (ALT) 35.56±1.16 iu/L and aspartate aminotransferase (AST) 44.16±1.83 iu/L were recorded for adult birds while alkaline phosphatase (ALP) values were significantly higher 104.86±16.39 iu/L in grower birds. Similarly, the rearing systems also influenced biochemical parameters of M. gallopavo and significantly (P<0.05) higher values for cholesterol 6.18±0.07 mmol/L and ALT 31.26±1.67 iu/L were recorded in birds reared under confinement rearing system. Values for globulin 1.92±0.36 g/dL and creatinin 0.77±0.08 μmol/L were higher in birds reared under semi-intensive rearing system while significantly higher urea concentrations 33.45±3.39 mg/dL were recorded from the serum of the birds reared under free range rearing system. However, non-significant variations in albumin, total protein, ALP and AST were recorded for all the three rearing systems. Significantly higher values of cholesterol, globulin, albumin, creatinine and ALT were recorded from the serum of male M. gallopavo while urea and ALP were higher in hens’ serum. However, non-significant variations were recorded for total protein and AST among both the genders. It can be concluded from the present study that age, gender and rearing systems strongly influence blood biochemical profile and hence the immunity in M. gallopavo. Availability: Items available for loan: UVAS Library [Call number: 2530-T] (1).

4. Comparative Studies On Egg Quality, Hematology And Reproductive Traits In Ring Necked And Green Pheasants In Captivity

by Qurat Ul Ain (2014-VA-963) | Dr. Arshad Javid | Dr. Ali Hussain | Prof. Dr. Muhammad Ashraf.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Present study was planned to compare egg quality, hematology and reproductive traits in ring necked and green pheasants in captivity. Day-old chicks of both the pheasant species Phasianuscolchicusand P.versicolorwere tagged individually and maintained initially in a room for a period of 4 weeks. The chicks were then transferred to cages provided with separate feeding and drinking facilities to the individual bird. The birds were kept until the 16 weeks of life. The sex of the chicks was predicted at early stages by observing feathers and plumage and was confirmed later at adult ages. Eggs (n = 100) of both the pheasant species i.e. Phasianuscolchicusand P.versicolorwere collected. Each egg was weighed and its length and breadth was taken. These eggs were divided into three weight groups and were classified as light, medium and heavy category. The length and breadth of each collected egg was taken and surface area, egg volume and shape index were calculated.The egg quality test was performed on freshly collected eggs in the egg quality testing laboratory periodically. The eggs were weighed carefully on electronic digital balance. The albumen and yolk height and width, yolk index, albumen and yolk pH and Haugh unit score were recorded. During present study, chick weight in ring necked pheasants Phasianuscolchicusand green pheasant P. versicolorfrom day old chick to 6-month stage varied significantly. The average body weight in day old chick weight ranged from20.6±1.35g to 24.50±1.29g.Increase in chick weight in male ring necked pheasants was 24.50±1.29g to 1235.25±101.81g. Similarly increase in female ring necked pheasant was 22.47±1.79gto 1004.75±52.94g.The chick average weight was almost double during 2nd week. Body length was maximum in male green pheasant 5.00±0.81cm during 1st week. However significant (p<0.05) increase in body weight was observed during 1st to 4thweek.Higher increase in average body weight was observed during 6thweek. Significantly (p<0.05) increase in wing length and wing span was also recorded during 6th week. During 7thweek, non-significant differences in body weight were observed between male and female P. colchicus.Overall, minimum increase in chick weight was observed during 21st,22nd and 23rd week and maximum during 6th,7th and 8th week of chick age. The chick weight at hatching in light, medium and heavy egg groups were determined as 19.5g, 21.8g and 22.6g, respectively. Lowest increase in chick body weight in green pheasants ranged from 20.6±1.35g during 1st week to 837.00±49.45g during 24thweek of its growth. During present study it was determined that hatched chick weight increases with increase in egg weight. After completion of the incubation, the infertile egg percentage was 48% in ring necked pheasant and 42% in green pheasant. Increase in wing length varied significantly in male and female and between both species from day old chick to 6-month stage. The lowest increase in chick’s wing length ranged from 5.37±1.10cm to 33.75±1.70cm in female P. versicolor. Overall minimum increase in wing length was observed during 12thweek and maximum during 2nd,3rd and 6th week of chick age. During present study, significant differences in various hematological parameters were recorded during different ages of pheasants. RBCs values in P.cholchicusincreased with age, reached a maximum point then decreased. While in P. versicolorthe values decreasedat juvenile stage and then increased to young ages and decreased. However, maximum 4.04±0.6 values for RBCs were recorded in P. versicolorduring 3rd month. In young age,significant (p<0.05) differences in blood biochemical profile of both the pheasant species were observed. Availability: Items available for loan: UVAS Library [Call number: 2674-T] (1).

5. Construction Of Cellulolytic And Sulfate-Reducing Bacterial Consortium For Enhancing Efficiency Of Cellulose-Linked Bioremedial Processes

by Ali Hasan (2014-VA-939) | Dr. Waseem Ahmad Khan | Dr. Arshad Javid | Prof. Dr. Muhammad Ashraf.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Metallic and non-metallic pollutants originating from different industries are not treated before their final discharge into the environment. Consequently, environment is being degraded very rapidly and posing serious threats to all forms of life. For remediation of the said pollutants, a number of physico-chemical treatment methods have been practiced but couldn’t found suitable due to environmentally non-compatible natures and generation of secondary pollutants. The present study was, therefore, designed to treat artificially prepared sulfate-rich wastewater jointly with the help of cellulolytic and sulfate-reducing bacterial species while using a variety of agro-industrial wastes as cost-effective growth substrates. In order to achieve the goal, the two bacterial species were mixed in different proportions to achieve significant results of sulfate reduction. Statistical analysis revealed that rice straw appeared as the most efficient carbon source among all the agricultural wastes because it reduced about 96% of the total added sulfate in a 60-day trial of anaerobic incubation. And among all the industrial wastes, animal manure appearedasthe most efficient carbon source, it could reduce 93% of sulfate. Mixture of industrial and agricultural waste reduced about 90% of the sulfate. Findings of this project will be helpful in developing an economical and environmental friendly bio-remedial technique for the treatment of metallic and non-metallic wastes simultaneously which ultimately convert the industrial wastewaters into harmless and suitable discharge to aquatic environment.   Availability: Items available for loan: UVAS Library [Call number: 2675-T] (1).



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